Check if your application supports 3D Mice. There is a list of such applications on the 3Dconnexion site.
Check the information provided by the editor of the application regarding 3D Mouse support.
Verify the 3D Mouse is enabled within your application. Some applications require specific steps to activate a 3D Mouse. Also, you may want to do a “repair” on your 3Dconnexion driver if it was installed before your application.